Bacteriology Media

Baird Parker Agar Base

Baird Parker Agar Base is used for the selective isolation and accurate enumeration of coagulase-positive Staphylococcus species.

Range	Bacteriology Media
Composition	Formula per liter of medium: Tryptone 10.00 gm; HM Peptone B# 5.00 gm; Yeast extract 1.00 gm; Glycine 12.00 gm; Sodium puruvate 10.00 gm; Lithium chloride 5.00 gm; Agar 20.00 gm. Final pH (at 25°C) 7.0 ± 0.2. ** Formula adjusted, standardized to suit performance parameters.
Preparation	Suspend 63.0 grams in 950 ml purified/ distilled water. Heat to boiling to dissolve the medium completely. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes. Cool to 50°C and aseptically add 50 ml concentrated Egg Yolk Emulsion (FD045) and 3 ml sterile PTe 3.5% Selective Supplement (1 ml per vial) (FD047) or 50 ml Egg Yolk Tel Emulsion (100 ml per vial) (FD046). For additional selectivity, if desired add rehydrated contents of 1 vial of BP S Selective Supplement (FD069). Alternatively 1 vial of FPT Inhibitor (FD195) may be used per 90 ml medium in place of Egg Yolk Tel Emulsion (100 ml per vial) (FD046) for identification of coagulase, positive Stapylococci. Mix well and pour into sterile Petri plates.
Storage	BioScien Baird Parker Agar should be stored between 10-30°C in a tightly closed container and the prepared medium at 2 - 8°C. Use before expiry date on the label. On opening, product should be properly stored dry, after tightly capping the bottle in order to prevent lump formation due to the hygroscopic nature of the product. Improper storage of the product may lead to lump formation. Store in dry ventilated area protected from extremes of temperature and sources of ignition. Seal the container tightly after use. Product performance is best if used within stated expiry period.
Appearance	The color of BioScien Baird Parker Agar Cream to yellow homogeneous free flowing powder. Basal medium: Yellow coloured clear to slightly opalescent gel. After addition of Egg Yolk Emulsion and Tellurite Emulsion: Yellow coloured opaque gel forms in Petri plates.
Pack sizes	3 options