Bacteriology Media
Bile Salt Agar
Bile Salt Agar is used for isolation and enumeration of bile tolerant enteric bacilli.
| Range | Bacteriology Media |
|---|---|
| Composition | Formula per liter of medium: Peptic digest of animal tissue 10.00 gm.; Meat extract 5.000 gm.; Sodium chloride 5.000 gm.; Sodium taurocholate 5.000 gm.; Agar 18.00 gm.; Final pH 8.2 ± 0.2 at 25°C |
| Preparation | Suspend 43 grams in 1000 ml distilled water. Adjust pH to 8.2 ± 0.2 at 25°C. Heat to boiling to dissolve the medium completely. Sterilize by autoclaving at 15 lbs. pressure (121°C) for 15 minutes. Pour into sterile petri plates. |
| Storage | BioScien Bile salt Agar should be stored between 10-30°C in a firmly closed container and the prepared medium at 2-8°C. Use before expiry date on the label. On opening, product should be properly stored dry, after tightly capping the bottle in order to avoid lump development due to the hygroscopic nature of the product. Improper storage of the product may lead to lump formation. Store in a dry ventilated area protected from extremes of temperature and sources of ignition. Seal the container tightly after use. Product performance is best if used within stated expiry period. |
| Appearance | The color of BioScien Bile salt agar is Cream to yellow homogeneous free flowing powder. Prepared Media is light amber coloured, clear to slightly opalescent gel forms in Petri plates. If there are any physical changes for powder or signs of deterioration (shrinking, cracking, or discoloration), and contaminations for hydrated media, discard the medium. |
| Specimen | Clinical samples |
| Pack sizes | 3 options |
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