Arena BioScien
Bacteriology Media
Bile Esculin Azide Agar
BS.1/BE01.100.0100
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Bacteriology Media
Bile Esculin Azide Agar
Bile Esculin Azide Agar is a selective medium used for isolation and presumptive identification of faecal Streptococci.
| Range | Bacteriology Media |
|---|---|
| Composition | Formula per liter of medium: Tryptone 17.00 gm; Beef extract 5.000 gm; Proteose peptone 3.000 gm; Ox gall 10.00 gm; Esculin 1.000 gm; Ferric ammonium citrate 0.500 gm; Sodium chloride 5.000 gm; Sodium azide 0.150 gm; Agar 15.00 gm. Final pH 7.1 ± 0.2 at 25°C |
| Preparation | Suspend 56.65 grams in 1000 ml distilled water. Heat to boiling to dissolve the medium completely. Sterilize by autoclaving at 15 lbs. pressure (121°C) for 15 minutes. Cool to 45-50°C. Mix well and pour into sterile Petri plates. |
| Storage | BioScien Bile Esculin Azide Agar are stable until expiration date stated on label when properly stored between 10-30°C in a tightly closed container and the prepared medium at 20-30°C. Use before expiry date on the label. On opening, product should be properly stored dry, after tightly capping the bottle in order to avoid lump development due to the hygroscopic nature of the product. Improper storage of the product may lead to lump formation. Store in a dry ventilated area protected from extremes of temperature and sources of ignition. Seal the container tightly after use. Product performance is best if used within stated expiry period. |
| Appearance | The color of BioScien Bile Esculin Azide Agar is cream to yellow coloured, homogeneous, free flowing powder. If there are any physical changes, discard the medium. The prepared medium is Amber coloured, clear to slightly opalescent gel with a bluish tinge forms in Petri plates, media should not be used if there are any signs of deterioration (shrinking, cracking, or discoloration), and contaminations. |
| Specimen | Food samples; Clinical samples |
| Pack sizes | 3 options |
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