Bacteriology Media
Bismuth Sulfite Agar
Recommended for the selective isolation of Salmonella from faeces, urine, sewage and other materials.
| Range | Bacteriology Media |
|---|---|
| Composition | Formula per liter of medium: Peptone 10; Beef extract 5; Dextrose 5; Disodium hydrogen phosphate, anhydrous 4; Ferrous sulphate 0.3; Bismuth sulphite indicator 8; Brilliant green 0.025; Agar 20. Final pH 7.7 ± 0.2 at 25°C |
| Preparation | Suspend 52.33 grams in 1000 ml distilled water. Adjust pH to 7.7 ± 0.2 at 25°C. Heat to boiling to dissolve the medium completely. DO NOT STERILIZE IN AUTOCLAVE or by fractional sterilization since overheating may destroy the selectivity of the medium. The sensitivity of the medium depends largely upon uniform dispersion of precipitated bismuth sulphite in the final gel, which should be dispersed before pouring into sterile Petri plates. |
| Storage | BioScien Bismuth Sulfite Agar dehydrated media are stable until expiration date stated on label when properly stored 10-30°C. The prepared medium should be stored at 2-8°C. Use before expiry date on the label. On opening, product should be properly stored dry, after tightly capping the bottle in order to avoid lump development due to the hygroscopic nature of the product. Improper storage of the product may lead to lump formation. Store in a dry ventilated area protected from extremes of temperature and sources of ignition. Seal the container tightly after use. Product performance is best if used within stated expiry period. |
| Appearance | The color of BioScien Bismuth Sulfite Agar is Light yellow to light green homogeneous free flowing powder. If there are any physical changes, discard the medium. Prepared medium is Greenish yellow coloured, opalescent with flocculent precipitate forms in Petri plates. Media should not be used if there are any signs of deterioration (shrinking, cracking, or discoloration), and contaminations. |
| Specimen | Clinical samples: faeces, urine, blood and other pathological material, Foodstuff, water sample |
| Pack sizes | 3 options |
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