Bacteriology Media
Brucella Selective Medium Base
Brucella Selective Medium Base is utilized for the enrichment, isolation, and identification of Brucella species.
| Range | Bacteriology Media |
|---|---|
| Composition | Concentration (g/L): Beef heart, infusion from 500g# 10.000; Tryptose 10.000; Sodium chloride 5.000; Gelatin 1.000; Dextrose (Glucose) 2.500; Agar 15.000; Final pH 7.2 ± 0.2 at 25°C |
| Preparation | Suspend 21.75 grams in 500 ml distilled water. Heat to boiling to dissolve the medium completely. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes. Cool to 45-50°C and aseptically add sterile 10 % v/v sheep blood and sterile 5% v/v inactivated horse serum (RM1239) (Inactivate RM1239 by heating at 56°C for 30 minutes). Also add rehydrated contents of one vial of Brucella Selective Supplement, (FD005). Mix well before pouring into sterile Petri plates. The prepared medium should be stored at 8-15°C. |
| Storage | BioScien Brucella Selective Medium Base media are stable until expiration date stated on label when properly stored at 10-30°C in a tightly closed container and the prepared medium at 8-15°C. Use before expiry date on the label. On opening, product should be properly stored dry, after tightly capping the bottle in order to prevent lump formation due to the hygroscopic nature of the product. Improper storage of the product may lead to lump formation. Store in dry ventilated area protected from extremes of temperature and sources of ignition. Seal the container tightly after use. Product performance is best if used within stated expiry period. |
| Appearance | BioScien Brucella Selective Medium Base medium is cream to yellow homogeneous free flowing powder. If there are any physical changes, discard the medium. Colour and Clarity of prepared medium: Light yellow coloured, clear to slightly opalescent gel forms in Petri plates. On addition of 10% v/v sterile sheep blood, cherry red coloured opalescent gel forms in Petri plates. |
| Specimen | Clinical samples: faeces |
| Pack sizes | 3 options |
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