Bacteriology Media
Buffered Peptone Water
Buffered Peptone Water is a pre-enrichment medium used for increasing the recovery of injured Salmonella species from foods prior to selective enrichment and isolation.
| Range | Bacteriology Media |
|---|---|
| Composition | Formula per liter of medium: Proteose peptone 10.000 gm.; Sodium chloride 5.000 gm.; Disodium phosphate, anhydrous 3.500 gm.; Monopotassium phosphate 1.500 gm.; Final pH 7.2 ± 0.2 at 25°C |
| Preparation | Suspend 20 grams in 1000 ml distilled water. Adjust pH to 7.2 ± 0.2 at 25°C. Heat to boiling to dissolve the medium completely. Sterilize by autoclaving at 15 lbs. pressure (121°C) for 15 minutes. If desired, aseptically add rehydrated contents of 1 vial of EC O157: H7 Selective Supplement for isolation of Escherichia coli O157 from foods to previously molten and cooled to 45-50°C medium. Pour into suitable containers. |
| Storage | BioScien Buffered Peptone Water should be stored between 10-30°C in a firmly closed container and the prepared medium at 2-8°C. Use before expiry date on the label. On opening, product should be properly stored dry, after tightly capping the bottle in order to avoid lump development due to the hygroscopic nature of the product. Improper storage of the product may lead to lump formation. Store in a dry ventilated area protected from extremes of temperature and sources of ignition. Seal the container tightly after use. Product performance is best if used within stated expiry period. |
| Appearance | The color of BioScien Buffered Peptone Water is Cream to yellow homogeneous free flowing powder. Prepared Media Light yellow coloured, clear solution without any precipitate. If there are any physical changes for powder or signs of deterioration (shrinking, cracking, or discoloration), and contaminations for hydrated media, discard the medium. |
| Specimen | Food and dairy samples |
| Pack sizes | 3 options |
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