Bacteriology Media

Esculin Fermentation Broth

Esculin Fermentation Broth is designed for cultivation and differentiation of bacteria which hydrolyze esculin.

RangeBacteriology Media
CompositionFormula per liter of medium: HM infusion B from # 500.00 gm; Tryptose 10.00 gm; Sodium chloride 5.00 gm; Esculin 1.00 gm; Agar 1.00 gm. Final pH (at 25oC) 7.0 ± 0.2. ** Formula adjusted, standardized to suit performance parameters.
PreparationSuspend 34.50 grams in 1000 ml purified / distilled water. Heat if necessary to dissolve the medium completely. Dispense into tubes or flasks as desired. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes. Note: Esculin hydolysis is observed on addition of Ferric citrate 0.1 gm/litr.
StorageBioScien Esculin Fermentation Broth Store between 10-30°C in a tightly closed container and the prepared medium at 15-25°C. Use before expiry date on the label. On opening, product should be properly stored dry, after tightly capping the bottle in order to prevent lump formation due to the hygroscopic nature of the product. Improper storage of the product may lead to lump formation. Store in dry ventilated area protected from extremes of temperature and sources of ignition. Seal the container tightly after use. Product performance is best if used within stated expiry period.
AppearanceThe color of BioScien Esculin Fermentation Broth Cream to yellow homogeneous free flowing powder. Prepared medium amber coloured, very slightly opalescent with purplish tinge.
Pack sizes3 options

Ordering information

Cat. No. (REF) Pack size Quote
BS.1/NZ01.100.0100 100 Gram Quote
BS.1/NZ01.250.0250 250 Gram Quote
BS.1/NZ01.500.0500 500 Gram Quote
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