Bacteriology Media
Giolitti Cantoni Broth Base
Giolitti Cantoni Broth Base is used for selective enrichment of Staphylococcus aureus from foods.
| Range | Bacteriology Media |
|---|---|
| Composition | Gram per liter of medium: Casein enzymic hydrolysate 10; Beef extract 5; Yeast extract 5; Mannitol 20; Sodium chloride 5; Lithium chloride 5; Glycerine 1.2; Sodium Pyruvate 3; Final pH 6.9 ± 0.2 at 25°C |
| Preparation | Suspend 54.2 grams in 1000 ml distilled water. Adjust pH to 6.9 ± 0.2 at 25°C. Warm gently to dissolve the medium completely. Dispense 19 ml amounts in 20mm x 200mm test tubes. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes. Cool rapidly to room temperature and aseptically add 0.3 ml of 3.5% Potassium Tellurite Solution to each tube. Add 0.03 ml for testing meat and meat products. Mix well before use. Caution: Lithium chloride is harmful. Avoid bodily contact and inhalation of vapours. On contact with skin, wash with plenty of water immediately. |
| Storage | BioScien Giolitti Cantoni broth base dehydrated media are stable until expiration date stated on label when properly stored 10-30°C. The prepared medium should be stored at 2-8°C. Use before expiry date on the label. On opening, product should be properly stored dry, after tightly capping the bottle in order to avoid lump development due to the hygroscopic nature of the product. Improper storage of the product may lead to lump formation. Store in a dry ventilated area protected from extremes of temperature and sources of ignition. Seal the container tightly after use. Product performance is best if used within stated expiry period. |
| Appearance | The color of BioScien Giolitti Cantoni broth base is Light yellow to light green homogeneous free flowing powder. If there are any physical changes, discard the medium. The hydrated medium is Medium amber colored, clear solution without any precipitate, media should not be used if there are any signs of deterioration (shrinking, cracking, or discoloration), and contaminations. |
| Specimen | Non-clinical sample |
| Pack sizes | 3 options |
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