Bacteriology Media
Gram Negative Enrichment Broth
Recommended for selective enrichment of gram-negative enteric organisms.
| Range | Bacteriology Media |
|---|---|
| Composition | Formula in g/L: Tryptose 20; Dextrose 1; Mannitol 2; Sodium citrate 5; Sodium deoxycholate 0.5; Dipotassium phosphate 4; Monopotassium phosphate 1.5; Sodium chloride 5. pH 7.0 ± 0.2 at 25°C |
| Preparation | Suspend 39 grams in 1000 ml distilled water. Heat if necessary to disssolve the medium completely. Dispense in test tubes. Sterilize by autoclaving at 115°C (10 lbs pressure) for 15 minutes. AVOID EXCESSIVE HEATING. |
| Storage | BioScien Gram Negative Enrichment Broth should be stored between 10-30°C in a firmly closed container and the prepared medium at 2-8°C. Use before expiry date on the label. On opening, product should be properly stored dry, after tightly capping the bottle in order to avoid lump development due to the hygroscopic nature of the product. Improper storage of the product may lead to lump formation. Store in a dry ventilated area protected from extremes of temperature and sources of ignition. Seal the container tightly after use. Product performance is best if used within the stated expiry period. |
| Appearance | BioScien Gram Negative Enrichment Broth is Cream to yellow homogeneous free flowing powder. Prepared medium is Light amber coloured, clear to slightly opalescent solution in tubes. Should not be used if there are any signs of deterioration (shrinking, cracking, or discoloration), and contaminations. |
| Specimen | Water Samples; Environmental samples |
| Pack sizes | 3 options |
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