Microbiology
Hektoen Entric Agar
Hektoen Enteric Agar is a differential selective medium designed for the selection of Shigella and Salmonella species from enteric pathological specimens.
| Range | Microbiology |
|---|---|
| Composition | gm / Liter: Proteose peptone 12.000; Yeast extract 3.000; Lactose 12.000; Sucrose 12.000; Salicin 2.000; Bile salts mixture 9.000; Sodium chloride 5.000; Sodium thiosulphate 5.000; Ferric ammonium citrate 1.500; Acid fuchsin 0.100; Bromothymol blue 0.065; Agar 15.000. Final pH (at 25°C) 7.5±0.2 |
| Preparation | Hydrated Hektoen Enteric agar media is prepared by suspend 76.67 grams in 1000 ml distilled water. Adjust pH to (at 25°C) 7.5±0.2. Heat to boiling to dissolve the medium completely. DO NOT AUTOCLAVE. Cool to 45-50°C. Mix well and pour into sterile Petri plates. |
| Storage | BioScien Hektoen Enteric agar dehydrated media are stable until expiration date stated on label when properly stored 10-30°C. Store the prepared medium at 20-30°C. Use before expiry date on the label. On opening, product should be properly stored dry, after tightly capping the bottle in order to prevent lump formation due to the hygroscopic nature of the product. Improper storage of the product may lead to lump formation. Store in dry ventilated area protected from extremes of temperature and sources of ignition. Seal the container tightly after use. |
| Appearance | The color of BioScien Hektoen Enteric Agar medium is cream to yellow with tancast homogeneous free flowing powder, dehydrated medium is green coloured to slightly opalescent gel. If there are any physical changes for powder or signs of deterioration (shrinking, cracking, or discoloration), and contaminations for hydrated media, discard the medium. |
| Specimen | Clinical samples: Blood, urine, faeces; Foods, water samples |
| Pack sizes | 3 options |
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