Bacteriology Media
Hoyel Medium Base
Hoyle Medium Base is a highly selective medium used for the isolation and differentiation of Corynebacterium diphtheriae types.
| Range | Bacteriology Media |
|---|---|
| Composition | Formula per liter of medium: Peptone 10.00 gm.; Beef extract 10.00 gm.; Sodium chloride 5.000 gm.; Agar 15.00 gm.; Final pH 7.8 ± 0.2 at 25°C |
| Preparation | Suspend 40.0 grams in 940 ml purified/distilled water. Adjust pH to 7.8 ± 0.2 at 25°C. Heat to boiling to dissolve the medium completely. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes. Cool to 45-50°C and aseptically add 50 ml of laked blood and 10 ml of 3.5% Potassium Tellurite Solution. Mix well and pour into sterile Petri plates. |
| Storage | BioScien Hoyel medium base should be stored between 10-30°C in a firmly closed container and the prepared medium at 2-8°C. Use before expiry date on the label. On opening, product should be properly stored dry, after tightly capping the bottle in order to avoid lump development due to the hygroscopic nature of the product. Improper storage of the product may lead to lump formation. Store in a dry ventilated area protected from extremes of temperature and sources of ignition. Seal the container tightly after use. Product performance is best if used within stated expiry period. |
| Appearance | The color of BioScien Hoyel medium base is Cream to yellow homogeneous free flowing powder. Prepared Basal Medium is Amber coloured, clear to slightly opalescent gel. After Addition of blood & Tellurite Brownish red coloured opaque gel forms in Petri plates. If there are any physical changes for powder or signs of deterioration (shrinking, cracking, or discoloration), and contaminations for hydrated media, discard the medium. |
| Specimen | Clinical samples - Blood; Food and dairy samples; Water samples |
| Pack sizes | 3 options |
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