Bacteriology Media
Lowenstein Jensen Medium Base (L.J. Medium)
Recommended for isolation and cultivation of Mycobacterium species.
| Range | Bacteriology Media |
|---|---|
| Composition | Formula per 600 ml of medium: L-Asparagine 3.600 gm; Potassium dihydrogen phosphate 2.400 gm; Magnesium sulphate 0.240 gm; Magnesium citrate 0.600 gm; Potato starch, soluble 30.00 gm; Malachite green 0.400 gm |
| Preparation | Suspend 37.24 grams in 600 ml purified / distilled water containing 12 ml glycerol (for bovine bacteria or other glycerophobic organisms additions of glycerol is not desirable). Heat if necessary to dissolve the medium completely. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes. Cool to 45-50°C. Meanwhile prepare 1000 ml of whole egg emulsion collected aseptically. Aseptically add and mix egg emulsion base and Gruft Mycobacterial Supplement (if desired) gently to obtain uniform mixture. Distribute in sterile screw capped tubes. Arrange tubes in a slanted position. Coagulate and inspissate the medium in an inspissator water bath or autoclave at 85°C for 45 minutes. |
| Storage | BioScien L.J. Medium should be stored between 10-30°C in a firmly closed container and the prepared medium at 2-8°C. Use before expiry date on the label. On opening, product should be properly stored dry, after tightly capping the bottle in order to avoid lump development due to the hygroscopic nature of the product. Improper storage of the product may lead to lump formation. Store in a dry ventilated area protected from extremes of temperature and sources of ignition. Seal the container tightly after use. Product performance is best if used within stated expiry period. |
| Appearance | The color of BioScien L.J. Medium is Greenish blue to peacock blue homogeneous free flowing powder. The mixture of sterile basal medium and whole egg emulsion, when inspissated, coagulates to yield pale bluish green coloured, opaque smooth slants. If there are any physical changes for powder or signs of deterioration (shrinking, cracking, or discoloration), and contaminations for hydrated media, discard the medium. |
| Specimen | Clinical samples: Sputum. |
| Pack sizes | 3 options |
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