Bacteriology Media
M-Lauryl Sulphate Broth
M-Lauryl Sulphate Broth is used for enumeration of Escherichia coli and coliforms in water, using membrane filter technique.
| Range | Bacteriology Media |
|---|---|
| Composition | Formula per liter of medium: Peptic digest of animal tissues 39.00 gm.; Yeast extract 6.000 gm.; Lactose 30.00 gm.; Phenol red 0.200 gm.; Sodium lauryl sulphate 1.000 gm.; Final pH 7.4 ± 0.2 at 25°C |
| Preparation | Suspend 76.2 grams in 1000 ml distilled water. Adjust pH to 7.4 ± 0.2 at 25°C. Heat if necessary to dissolve the medium completely. Dispense as desired and sterilize by steaming for 30 minutes on three consecutive days or by autoclaving at 15 lbs pressure (121°C) for 15 minutes. |
| Storage | BioScien M-Lauryl Sulphate Broth dehydrated media are stable until expiration date stated on label when properly stored 10-30°C. The prepared medium should be stored at 2-8°C. Use before expiry date on the label. On opening, product should be properly stored dry, after tightly capping the bottle in order to avoid lump development due to the hygroscopic nature of the product. Improper storage of the product may lead to lump formation. Store in a dry ventilated area protected from extremes of temperature and sources of ignition. Seal the container tightly after use. Product performance is best if used within stated expiry period. |
| Appearance | The color of BioScien M-Lauryl Sulphate Broth is Light yellow to pink homogeneous free flowing powder. If there are any physical changes, discard the medium. The prepared medium is Red coloured clear solution without any precipitate, media should not be used if there are any signs of deterioration (shrinking, cracking, or discoloration), and contaminations. |
| Specimen | Clinical samples |
| Pack sizes | 3 options |
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