Bacteriology Media

MacConkey Agar without Crystal Violet

Recommended for the cultivation and differentiation of enteric bacteria, restricting swarming of Proteus species from specimens such as urine which may contain large number of Proteus species as well as potentially pathogenic Gram positive organisms.

Range	Bacteriology Media
Composition	Ingredient g\L: Peptone 20.000; Lactose 10.000; Sodium taurocholate 5.000; Neutral red 0.040; Agar 20.000; Final pH 7.4 ± 0.2 at 25°C
Preparation	Hydrated MacConkey Agar without Crystal Violet media is Suspend 55.0 grams of medium in 1000 ml purified/distilled water. Heat to boiling with gentle swirling to dissolve the agar completely. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes. Avoid overheating. Cool to 45-50°C. Mix well and pour into sterile Petri plates. The surface of the medium should be dry when inoculated.
Storage	BioScien MacConkey Agar without Crystal Violet dehydrated media are stable until expiration date stated on label when properly stored 10-30°C. Store between 10-30°C in a tightly closed container and the prepared medium at 20-30°C. Use before expiry date on the label. On opening, product should be properly stored dry, after tightly capping the bottle in order to prevent lump formation due to the hygroscopic nature of the product. Improper storage of the product may lead to lump formation. Store in dry ventilated area protected from extremes of temperature and sources of ignition Seal the container tightly after use. Product performance is best if used within stated expiry period.
Appearance	The color of BioScien MacConkey Agar without Crystal Violet Light yellow to pink homogeneous free flowing powder, dehydrated medium Orange red coloured clear to slightly opalescent gel forms in Petri plates. If there are any physical changes for powder or signs of deterioration (shrinking, cracking, or discoloration), and contaminations for hydrated media, discard the medium.
Specimen	Water, Faeces, Urine, Food and Dairy samples
Pack sizes	3 options