Arena BioScien
Bacteriology Media
M-Endo Agar LES
BS.1/NZ01.100.0100
Fits your perfection
Bacteriology Media
M-Endo Agar LES
M - Endo Agar LES is designed for enumerating coliforms in water by membrane filtration.
| Range | Bacteriology Media |
|---|---|
| Composition | Formula per liter of medium: peptone 3.7 gm; Trypton 3.7 gm; Tryptose 7.5 gm; Lactose 9.4 gm; Yeast extract 1.2 gm; potassium dihydrogen phosphate 1.0 gm; dipotassium dihydrogen phosphate 3.3 gm; Basic fuchsin 0.8 gm; Sodium chloride 3.7 gm; Sodium deoxycholate 0.1 gm; Sodium sulphite anhydrus 1.6 gm; Sodium lauryl sulphate 0.05 gm; Agar 15.0 gm. Final pH 7.2 ± 0.2 |
| Preparation | Suspend 51.05 grams in 980 ml purified/distilled water. Heat to boiling to dissolve the medium completely. DO NOT AUTOCLAVE. Cool to 45-50°C and aseptically add 20 ml of 95% ethanol. Mix and dispense 4 ml amounts into 60 mm Petri plates. In large plates, use sufficient medium to give 1.5 mm depth. DO NOT EXPOSE PLATES TO DIRECT SUNLIGHT. |
| Storage | BioScien M-Endo Agar LES Store between 10-30°C in a tightly closed container and the prepared medium at 15-25°C. Use before expiry date on the label. On opening, product should be properly stored dry, after tightly capping the bottle in order to prevent lump formation due to the hygroscopic nature of the product. Improper storage of the product may lead to lump formation. Store in dry ventilated area protected from extremes of temperature and sources of ignition. Seal the container tightly after use. Product performance is best if used within stated expiry period. |
| Appearance | The color of BioScien Purple, homogeneous free flowing powder. |
| Pack sizes | 3 options |
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