Bacteriology Media

MacConkey Sorbitol Agar

MacConkey Sorbitol Agar is used for isolation and identification of enteropathogenic Escherichia coli strains associated with infant diarrhea.

Range	Bacteriology Media
Composition	Formula in g/L: Peptone 17; Protese peptone 3; D-Sorbitol 10; Bile salts mixture 1.5; Sodium chloride 5; Neutral red 0.03; Crystal violet 0.001; Agar 13.5; pH 7.1 ± 0.2 at 25°C
Preparation	Suspend 50.0 grams in 1000 ml purified / distilled water. Adjust pH to pH 7.1 ± 0.2 at 25°C. Heat to boiling to dissolve the medium completely. Sterilize by autoclaving at 15 lbs. pressure (121°C) for 15 minutes. Mix well and pour into sterile Petri plates.
Storage	BioScien MacConkey Sorbitol Agar should be stored between 10-30°C in a firmly closed container and the prepared medium at 2-8°C. Use before expiry date on the label. On opening, product should be properly stored dry, after tightly capping the bottle in order to avoid lump development due to the hygroscopic nature of the product. Improper storage of the product may lead to lump formation. Store in a dry ventilated area protected from extremes of temperature and sources of ignition. Seal the container tightly after use. Product performance is best if used within stated expiry period.
Appearance	BioScien MacConkey Sorbitol Agar is Light yellow to pink homogeneous free flowing powder. Prepared medium is Purplish red coloured clear to slightly opalescent gel forms in Petri plates. Media should not be used if there are any signs of deterioration (shrinking, cracking, or discoloration), and contaminations.
Specimen	For clinical samples follow appropriate techniques for handling specimens as per established guidelines (11,12). For food and dairy samples, follow appropriate techniques for sample collection and processing as per guidelines (9,10,13). After use, contaminated materials must be sterilized by autoclaving before discarding. Type of specimen: Clinical samples - stool; Food and Dairy samples
Pack sizes	3 options