Microbiology
MacConkey Broth Purple
MacConkey Broth Purple w/BCP is used for presumptive identification of coliforms from variety of specimens such as water, milk, food and clinical samples.
| Range | Microbiology |
|---|---|
| Composition | Formula per liter of medium: Peptone 10; Bile salt 4; Sodium chloride 5; Lactose 30; Bromocresol purple 0.12; Final pH 7.4 ± 0.2 at 25°C |
| Preparation | Suspend 49.12 grams in 1000 ml distilled water. Adjust pH to 7.4 ± 0.2 at 25°C. Heat to boiling to dissolve the medium completely. Distribute into tubes with inverted Durham tubes and sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes. |
| Storage | BioScien MacConkey broth dehydrated media are stable until expiration date stated on label when properly stored 10-30°C. The prepared medium should be stored at 2-8°C. Use before expiry date on the label. On opening, product should be properly stored dry, after tightly capping the bottle in order to avoid lump development due to the hygroscopic nature of the product. Improper storage of the product may lead to lump formation. Store in a dry ventilated area protected from extremes of temperature and sources of ignition. Seal the container tightly after use. Product performance is best if used within stated expiry period. |
| Appearance | The color of BioScien MacConkey broth is Cream to yellow homogeneous free flowing powder. If there are any physical changes, discard the medium. The hydrated medium is Purple coloured clear solution without any precipitate, media should not be used if there are any signs of deterioration (shrinking, cracking, or discoloration), and contaminations. |
| Specimen | Food samples; Water and sewage samples |
| Pack sizes | 3 options |
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