Bacteriology Media

Mannitol Lysine Crystal Violet Brilliant Green Agar

Mannitol Lysine Agar is used for selective isolation of Salmonella species other than Salmonella Typhi and Salmonella Paratyphi A.

Range	Bacteriology Media
Composition	Formula in g/L: Peptic digest of animal tissue 10; Yeast extract 5; Beef extract 2; Sodium chloride 4; Mannitol 3; L-lysine 5; Sodium thiosulphate 4; Ferric ammonium citrate 1; Brilliant green 0.0125; Crystal violet 0.01; Agar 15; pH 6.8 ± 0.2 at 25°C
Preparation	Suspend 49.02 grams in 1000 ml distilled water. Heat to boiling to dissolve the medium completely. DO NOT AUTOCLAVE. Cool to 45-50°C. Mix well and pour into sterile Petri plates.
Storage	BioScien Mannitol Lysine Crystal Violet Brilliant Green Agar should be stored between 10-30°C in a firmly closed container and the prepared medium at 2-8°C. Use before expiry date on the label. On opening, product should be properly stored dry, after tightly capping the bottle in order to avoid lump development due to the hygroscopic nature of the product. Improper storage of the product may lead to lump formation. Store in a dry ventilated area protected from extremes of temperature and sources of ignition. Seal the container tightly after use. Product performance is best if used within the stated expiry period.
Appearance	BioScien Mannitol Lysine Crystal Violet Brilliant Green Agar is Cream to yellow homogeneous free flowing powder. Prepared medium is Light amber coloured, opalescent gel with flocculent precipitate forms in tubes as slants. should not be used if there are any signs of deterioration (shrinking, cracking, or discoloration), and contaminations.
Specimen	For clinical samples follow appropriate techniques for handling specimens as per established guidelines (11,12). For food and dairy samples, follow appropriate techniques for sample collection and processing as per guidelines (9,10,13). After use, contaminated materials must be sterilized by autoclaving before discarding. Type of specimen: Clinical samples; Food and Dairy samples; Environmental samples
Pack sizes	3 options