Bacteriology Media

Simmons Citrate Agar

Simmons Citrate Agar is designed for differentiation between faecal coli and members of the aerogenes group based on citrate utilization.

Range	Bacteriology Media
Composition	Formula per liter of medium: Ammonium dihydrogen phosphate 1.00 gm; Magnesium sulphate 0.200 gm; Dipotassium phosphate 1.00 gm; Sodium citrate 2.00 gm; Sodium chloride 5.00 gm; Bromo thymol blue 0.08 gm; Agar 15.00 gm. Final pH 6.8 ± 0.2. ** Formula adjusted, standardized to suit performance parameters.
Preparation	Suspend 24.28 grams in 1000 ml distilled water. Heat to boiling to dissolve the medium completely. Mix well and distribute in tubes. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes. Cool the tubes as slants.
Storage	BioScien Simmons Citrate Store between 10-30°C in a tightly closed container and the prepared medium at 15-25°C. Use before expiry date on the label. On opening, product should be properly stored dry, after tightly capping the bottle in order to prevent lump formation due to the hygroscopic nature of the product. Improper storage of the product may lead to lump formation. Store in dry ventilated area protected from extremes of temperature and sources of ignition. Seal the container tightly after use. Product performance is best if used within stated expiry period.
Appearance	The color of BioScien Simmons Citrate agar Is yellow coloured homogeneous free flowing powder. Prepared medium is Forest green coloured slightly opalescent gel forms as slants.
Pack sizes	3 options