Bacteriology Media
Shigella Broth
Shigella Broth Base is used for the isolation and cultivation of Shigella species from food.
| Range | Bacteriology Media |
|---|---|
| Composition | Formula per liter of medium: Casein enzymic hydrolysate 20 gm; Sodium chloride 5 gm; Dipotassium hydrogen phosphate 2 gm; Potassium dihydrogen phosphate 2 gm; Dextrose 1 gm; Final pH 7.0 ± 0.2 at 25°C |
| Preparation | Suspend 31.5 grams in 1000 ml distilled water. Add Polysorbate 80 to the medium. Adjust pH to 7.0 ± 0.2 at 25°C. Heat if necessary to dissolve the medium completely. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes. Cool medium to 45-50°C and add rehydrated content of 1 vial of Novobiocin supplement under aseptic conditions. Mix well and dispense in sterile test tubes. |
| Storage | BioScien Shigella Broth should be stored between 10-30°C in a firmly closed container and the prepared medium at 2-8°C. Use before expiry date on the label. On opening, product should be properly stored dry, after tightly capping the bottle in order to avoid lump development due to the hygroscopic nature of the product. Improper storage of the product may lead to lump formation. Store in a dry ventilated area protected from extremes of temperature and sources of ignition. Seal the container tightly after use. Product performance is best if used within stated expiry period. |
| Appearance | The color of BioScien Shigella Broth is Cream to yellow homogeneous free flowing powder. Prepared Media is Light amber coloured clear solution. If there are any physical changes for powder or signs of deterioration (shrinking, cracking, or discoloration), and contaminations for hydrated media, discard the medium. |
| Specimen | Clinical samples (e.g., faeces, blood, and wounds). Food sample (milk and other dairyproducts) |
| Pack sizes | 3 options |
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