Bacteriology Media

Sulfur Indole Motility Medium

Semisolid medium used for the differentiation between microorganisms based on hydrogen sulfide production, indole production, and motility.

Range	Bacteriology Media
Composition	Formula per liter of medium: Tryptone 20 gm; Peptone 6.1 gm; Ferrous ammonium sulphate 0.2 gm; Sodium thiosulfate 0.2 gm; Agar 3.5 gm; Final pH 7.3 ± 0.2 at 25°C
Preparation	Suspend 30 grams in 1-liter distilled water. Heat to boiling to dissolve the medium completely. Distribute in test tubes. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes. Allow the tubes to cool in a vertical position.
Storage	BioScien SIM Medium should be stored between 10-30°C in a firmly closed container and the prepared medium at 2-8°C. Use before expiry date on the label. On opening, product should be properly stored dry, after tightly capping the bottle in order to avoid lump development due to the hygroscopic nature of the product. Improper storage of the product may lead to lump formation. Store in a dry ventilated area protected from extremes of temperature and sources of ignition. Seal the container tightly after use. Product performance is best if used within stated expiry period.
Appearance	The color of BioScien SIM Medium is cream to beige homogeneous free flowing powder. Prepared Media is light amber in color. If there are any physical changes for powder or signs of deterioration (shrinking, cracking, or discoloration), and contaminations for hydrated media, discard the medium.
Specimen	Clinical and non-clinical specimens.
Pack sizes	3 options