Bacteriology Media
Thayer Martin Medium Base
Recommended for selective isolation of Gonococci from pathological specimens.
| Range | Bacteriology Media |
|---|---|
| Composition | Formula per liter of medium: Peptone, Special 23.00 gm; Starch 1.00 gm; Sodium chloride 5.00 gm; Agar 13.00 gm; Final pH 7.0 ± 0.2 at 25°C |
| Preparation | Suspend 21.0 grams in 450 ml distilled water. Heat to boiling to dissolve the medium completely. Sterilize by autoclaving at 15 Lbs. pressure (121°C) for 15 minutes. Cool to 45°C. Aseptically add 50 ml of sterile lysed blood and rehydrated contents of one vial of Vitamino Growth Supplement and V.C.N Supplement or V.C.N.T Supplement. If desired GC Supplement with Antibiotics can be used as a single supplement. Mix well before pouring into sterile Petri plates. If Hemoglobin is used suspend 21.0 grams of Thayer Martin Medium Base in 250 ml distilled water. Heat to boiling to dissolve the medium completely. Prepare 250 ml of 2% hemoglobin solution. Sterilize separately by autoclaving at 15 lbs. pressure (121°C) for 15 minutes. Cool to 45°C. Mix both and add the supplements as above. |
| Storage | BioScien Thayer Martin Medium Base are stable until expiration date stated on label when properly stored 10-30°C. The prepared medium should be stored at 2-8°C. Use before expiry date on the label. On opening, product should be properly stored dry, after tightly capping the bottle in order to avoid lump development due to the hygroscopic nature of the product. Improper storage of the product may lead to lump formation. Store in a dry ventilated area protected from extremes of temperature and sources of ignition. Seal the container tightly after use. Product performance is best if used within stated expiry period. |
| Appearance | The color of BioScien Thayer Martin Medium Base is Cream to yellow homogeneous free flowing powder. If there are any physical changes, discard the medium. The hydrated medium is (Basal Medium) Yellow coloured clear to slightly opalescent gel. After addition of hemoglobin or sterile lysed blood and supplements: chocolate coloured opaque gel forms in Petri plates, media should not be used if there are any signs of deterioration (shrinking, cracking, or discoloration), and contaminations. |
| Specimen | Clinical samples. |
| Pack sizes | 3 options |
More in this range
View the full range
Bacteriology Media
IFU
Thioglycollate Agar
- 3 pack sizes
IFU
Thioglycollate Broth
- 3 pack sizes
IFU
Thioglycollate Medium w/o Dextrose
- 3 pack sizes
Thiosulfate Citrate Bile Salts Sucrose Agar (TCBS Agar)
- 1 pack size
IFU
Tinsdale Agar Base
- 3 pack sizes
IFU
Todd Hewitt Broth
- 3 pack sizes
IFU
Tributyrin Agar
- 3 pack sizes
IFU
Triple Sugar Iron Agar
- 3 pack sizes
Tryptic Soy Agar
- 1 pack size
Tryptic Soy Broth
- 1 pack size
Let’s equip your laboratory.
Tell us what your laboratory needs — our team will get back to you with product information, availability and a quotation.