Bacteriology Media
Tetrathionate Broth
Used with iodine for the selective enrichment of salmonellae from various materials.
| Range | Bacteriology Media |
|---|---|
| Composition | Formula per liter of medium: Enzymatic Digest of Casein 2.500 gm.; Enzymatic Digest of Animal Tissue 2.500 gm.; Bile Salts 1.000 gm.; Calcium Carbonate 10.00 gm.; Sodium Thiosulfate 30.00 gm.; Final pH 8.4 ± 0.2 at 25°C |
| Preparation | Dissolve 46 g of the medium in one liter of purified water. Adjust pH to 8.4 ± 0.2 at 25°C. Heat with frequent agitation and boil for one minutes to completely dissolve the medium. Cool to 45-50°C and add 20 mL of the Iodine-Potassium Iodide Solution to the prepared Tetrathionate Broth Base. If preparing solution, add 6 grams Iodine + 5 grams Potassium Iodide in 20 mL of purified water. DO NOT REHEAT AFTER ADDING IODINE SOLUTION. Note: Do not add Iodine/Potassium Iodide Solution to tubes until just before inoculation. Chemical Tetrathionate inhibits by oxidation of Thiosulfate through the addition of Iodine just prior to use. |
| Storage | BioScien Tetrathionate broth should be stored between 10-30°C in a firmly closed container and the prepared medium at 2-8°C. Use before expiry date on the label. On opening, product should be properly stored dry, after tightly capping the bottle in order to avoid lump development due to the hygroscopic nature of the product. Improper storage of the product may lead to lump formation. Store in a dry ventilated area protected from extremes of temperature and sources of ignition. Seal the container tightly after use. Product performance is best if used within stated expiry period. |
| Appearance | The color of BioScien Tetrathionate broth is white to off-white homogeneous free flowing powder. Prepared Medium is Prepared medium is milky white to slightly yellow-white and opaque solution forms in tubes. If there are any physical changes for powder or signs of deterioration (shrinking, cracking, or discoloration), and contaminations for hydrated media, discard the medium. |
| Specimen | Clinical samples; Food; Water |
| Pack sizes | 3 options |
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