Bacteriology Media
Thioglycollate Broth
Recommended for the recovery of anaerobic and facultative microorganisms.
| Range | Bacteriology Media |
|---|---|
| Composition | Formula per liter of medium: Tryptone 15.00 gm.; Yeast Extract 5.000 gm.; D(+)-Glucose 5.500 gm.; L-Cystine 0.500 gm.; Sodium chloride 2.500 gm.; Sodium Thioglycollate 0.500 gm.; Final pH 7.1 ± 0.2 at 25°C |
| Preparation | Suspend 29 grams in 1000 ml distilled water. Adjust pH to 7.1 ± 0.2 at 25°C. Dispense into tubes. Autoclave 15 min at 121 °C. |
| Storage | BioScien Thioglycollate broth should be stored between 10-30°C in a firmly closed container and the prepared medium at 2-8°C. Use before expiry date on the label. On opening, product should be properly stored dry, after tightly capping the bottle in order to avoid lump development due to the hygroscopic nature of the product. Improper storage of the product may lead to lump formation. Store in a dry ventilated area protected from extremes of temperature and sources of ignition. Seal the container tightly after use. Product performance is best if used within stated expiry period. |
| Appearance | The color of BioScien Thioglycollate broth base is Cream to yellow homogeneous free flowing powder. Prepared Media Light yellow coloured clear solution. If there are any physical changes for powder or signs of deterioration (shrinking, cracking, or discoloration), and contaminations for hydrated media, discard the medium. |
| Specimen | Clinical sample: Blood. |
| Pack sizes | 3 options |
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