Bacteriology Media
Bordet Gengou Agar Base
Is used for the detection and isolation of Bordetella pertussis and Bordetella parapertussis.
| Range | Bacteriology Media |
|---|---|
| Composition | Formula per liter of medium: Potatoes infusion from 125 g 4.5 gm.; Peptone 10.00 gm.; Sodium chloride 5.5 gm.; Agar 20.0 gm. Final pH (at 25°C) 6.7 ± 0.2 |
| Preparation | Bordet Gengou agar media is Suspend 40.00 grams in 1000 ml distilled water containing 10 ml glycerol. Heat to boiling to dissolve the medium completely. Sterilize by autoclaving at 15 lbs. pressure (121°C) for 15 minutes. Cool to 45-50°C and aseptically add 15 - 20% sterile, fresh defibrinated blood (sheep, rabbit, human or horse). For selectivity aseptically add rehydrated contents of 2 vials of Bordetella Selective supplement (FD004). Mix thoroughly, taking care to avoid incorporation of air bubbles and pour into sterile Petri plates. |
| Storage | BioScien Bordet Gengou agar media are stable until expiration date stated on label when properly stored at 10-30°C. |
| Appearance | BioScien Bordet Gengou is Cream to yellow to coloured homogeneous free flowing powder. If there are any physical changes, discard the medium. Prepared medium appearance: Basal Medium: Light yellow colored clear to slightly opalescent gel. After addition of glycerol and 15% v/v sterile defibrinated blood: Cherry red coloured opaque gel forms in Petri plates. Media should not be used if there are any signs of deterioration (shrinking, cracking, or discoloration), and contaminations. |
| Specimen | Clinical samples |
| Pack sizes | 3 options |
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