Bacteriology Media

Bolton Broth Base

Bolton Broth Base is a selective enrichment medium used for the isolation and recovery of Campylobacter species from food and animal feed samples, as well as thermotolerant Campylobacter spp. from water samples.

Range	Bacteriology Media
Composition	Formula per liter of medium: Peptone 10 gm; Lactalbumin hydrolysate 5.0 gm; Yeast extract 5.0 gm; Sodium chloride 5.0 gm; Sodium metabisulphite 0.5 gm; Sodium carbonate 0.6 gm; Hemin 0.01 gm; α-ketoglutaric acid 1.0 gm; Sodium pyruvate 0.5 gm. Final pH (at 25oC) 7.4 ± 0.2. ** Formula adjusted, standardized to suit performance parameters.
Preparation	Suspend 13.80 gram in 500 ml purified/distilled water. Heat if necessary to dissolve the medium completely. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes. Cool to 45-50°C and aseptically add rehydrated contents of 1 vial of Bolton Selective supplement (FD231) and 25 ml of sterile lysed defibrinated horse blood in the medium. Horse blood may be saponin lysed or lysed by freezing then thawing out. Mix well and aseptically dispense into sterile tubes or flasks as desired.
Storage	BioScien Bolton Broth Base Store between 10-30°C in a tightly closed container and the prepared medium at 15-25°C. Use before expiry date on the label. On opening, product should be properly stored dry, after tightly capping the bottle in order to prevent lump formation due to the hygroscopic nature of the product. Improper storage of the product may lead to lump formation. Store in dry ventilated area protected from extremes of temperature and sources of ignition. Seal the container tightly after use. Product performance is best if used within stated expiry period.
Appearance	The color of BioScien Bolton Broth Base Light yellow to brownish yellow homogeneous free flowing powder. Basal medium: Brownish yellow coloured clear to slightly opalescent solution. After addition of lysed horse blood: Red to brown coloured opaque solution in tubes.
Pack sizes	3 options