Bacteriology Media
Brain Heart Infusion (BHI) Agar
Designed for cultivation of fastidious pathogenic bacteria, yeasts and moulds from clinical and non clinical samples.
| Range | Bacteriology Media |
|---|---|
| Composition | Formula per liter of medium: Glucose (Dextrose) 2.00 gm; Sodium chloride 5.00 gm; Disodium hydrogen phosphate 2.5 gm; BHI powder 5.00 gm; Peptone 10.0; HM infusion powder # 12.5; Agar 15. Final pH 7.4 ± 0.2. # Equivalent to Dehydrated calf brain infusion. ** Formula adjusted, standardized to suit performance parameters. |
| Preparation | Suspend 52.0 grams in 1000 ml purified/distilled water. Heat to boiling to dissolve the medium completely. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes. Cool to 45-50°C. Mix well and pour into sterile Petri plates. If desired, 20 units Penicillin and 40 µg Streptomycin per ml of medium may be added to make the medium selective for fungi. |
| Storage | BioScien BHI agar Store between 10-30°C in a tightly closed container and the prepared medium at 15-25°C. Use before expiry date on the label. On opening, product should be properly stored dry, after tightly capping the bottle in order to prevent lump formation due to the hygroscopic nature of the product. Improper storage of the product may lead to lump formation. Store in dry ventilated area protected from extremes of temperature and sources of ignition. Seal the container tightly after use. Product performance is best if used within stated expiry period. |
| Appearance | The color of BioScien BHI agar is Cream to yellow homogeneous free flowing powder. Basal medium: Light amber coloured clear to slightly opalescent gel. After addition of 5% v/v sterile defibrinated blood: Cherry red coloured, opaque gel forms in Petri plates. |
| Pack sizes | 3 options |
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