Bacteriology Media
Brucella Broth Base
Brucella Broth Base with supplement is recommended for enrichment and cultivation of Brucella or Campylobacter species from clinical and non-clinical specimens.
| Range | Bacteriology Media |
|---|---|
| Composition | Formula per liter of medium: Peptone 10.00 gm.; Tryptone 10.00 gm.; Yeast extract 2.000 gm.; Dextrose (Glucose) 1.000 gm.; Sodium chloride 5.000 gm; Sodium bisulphite 0.1 gm; Final pH 7.0 ± 0.2 at 25°C |
| Preparation | Suspend 14.05 grams in 500 ml distilled water. Adjust pH to 7.0 ± 0.2 at 25°C. Heat if necessary to dissolve the medium completely. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes. Cool to 45-50°C and aseptically add sterile 5% v/v inactivated horse serum (Inactivate by heating at 56°C for 30 minutes). Add rehydrated contents of one vial of Brucella Selective Supplement. Mix well before pouring into sterile tubes. For Campylobacter: Aseptically add sterile rehydrated contents of 1 vial of Campylobacter Supplement I (Blaser Wang) or Campylobacter Supplement II (Butzler) or Campylobacter Supplement III (Skirrow) and Campylobacter Growth Supplement to 500 ml of sterile medium. |
| Storage | BioScien Brucella broth base should be stored between 10-30°C in a firmly closed container and the prepared medium at 2-8°C. Use before expiry date on the label. On opening, product should be properly stored dry, after tightly capping the bottle in order to avoid lump development due to the hygroscopic nature of the product. Improper storage of the product may lead to lump formation. Store in a dry ventilated area protected from extremes of temperature and sources of ignition. Seal the container tightly after use. Product performance is best if used within stated expiry period. |
| Appearance | The color of BioScien Brucella broth base is Cream to yellow homogeneous free flowing powder. Prepared Media is Light amber coloured, clear solution in tubes. If there are any physical changes for powder or signs of deterioration (shrinking, cracking, or discoloration), and contaminations for hydrated media, discard the medium. |
| Specimen | Clinical: faeces |
| Pack sizes | 3 options |
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