Bacteriology Media
Iron Sulphite Agar
Iron Sulphite Agar is recommended for the detection of thermophilic anaerobic organisms causing sulphide spoilage in food.
| Range | Bacteriology Media |
|---|---|
| Composition | Formula per liter of medium: Casein enzymic hydrolysate 10; Sodium sulphite 0.5; Ferric citrate 0.5; Agar 15; Final pH 7.1 ± 0.2 at 25°C |
| Preparation | Suspend 26 grams in 1000 ml distilled water. Adjust pH to 7.1 ± 0.2 at 25°C. Heat to boiling to dissolve the medium completely. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes. Mix well and pour into sterile Petri plates. |
| Storage | BioScien Iron Sulphite Agar dehydrated media are stable until expiration date stated on label when properly stored 10-30°C. The prepared medium should be stored at 2-8°C. Use before expiry date on the label. On opening, product should be properly stored dry, after tightly capping the bottle in order to avoid lump development due to the hygroscopic nature of the product. Improper storage of the product may lead to lump formation. Store in a dry ventilated area protected from extremes of temperature and sources of ignition. Seal the container tightly after use. Product performance is best if used within stated expiry period. |
| Appearance | The color of BioScien Iron Sulphite Agar Light yellow to brownish yellow homogeneous free flowing powder. If there are any physical changes, discard the medium. The hydrated medium is Yellow coloured, slightly opalescent gel forms in Petri plates, media should not be used if there are any signs of deterioration (shrinking, cracking, or discoloration), and contaminations. |
| Specimen | Food samples; Water and sewage samples |
| Pack sizes | 3 options |
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