Bacteriology Media

Kligler Iron Agar

Designed for differential identification of gram-negative enteric bacilli on the basis of the fermentation of glucose (dextrose), lactose and hydrogen sulphide production.

Range	Bacteriology Media
Composition	Formula per liter of medium: Peptone 15 gm; Beef Extract 3 gm; Yeast Extract 3 gm; Tryptone 5 gm; Lactose 10 gm; Dextrose 1 gm; Ferrous sulphate 0.2 gm; Sodium chloride 5 gm; Sodium thiosulphate 0.3 gm; Phenol red 0.024 gm; Agar 15 gm. Final pH 7.4±0.2 at 25°C
Preparation	Suspend 57.52 grams in 1000 ml distilled water. Heat to boiling to dissolve the medium completely. Mix well and distribute into sterile test tubes. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes. Allow the tubes to cool in slanted position to form slopes with about 1 inch butts. Best reactions are obtained on freshly prepared medium. Do not use screw capped tubes or bottles.
Storage	BioScien Kligler Iron Agar should be stored between 10-30°C in a firmly closed container and the prepared medium at 2-8°C. Use before expiry date on the label. On opening, product should be properly stored dry, after tightly capping the bottle in order to avoid lump development due to the hygroscopic nature of the product. Improper storage of the product may lead to lump formation. Store in a dry ventilated area protected from extremes of temperature and sources of ignition. Seal the container tightly after use. Product performance is best if used within stated expiry period.
Appearance	The color of BioScien Kligler Iron Agar medium is Light yellow to pink homogeneous free flowing powder. Prepared Media is reddish orange in color. If there are any physical changes for powder or signs of deterioration (shrinking, cracking, or discoloration), and contaminations for hydrated media, discard the medium.
Specimen	Clinical samples, water samples, food and dairy samples.
Pack sizes	3 options