Bacteriology Media
Loeffler Serum Medium Base
Recommended for the cultivation of Corynebacterium diphtheriae.
| Range | Bacteriology Media |
|---|---|
| Composition | Formula per liter of medium: Heart muscle, infusion from 0.720 gm; Peptone 0.710 gm; Sodium chloride 0.360 gm; Dextrose (Glucose) 0.710 gm; Egg powder 7.500 gm; Final pH 7.6±0.2 at 25°C |
| Preparation | Suspend 10.0 grams in 250 ml purified / distilled water at a temperature of 45°C. Add 750 ml of sterile Bovine serum, mix well and dispense into tubes. Coagulate and sterilize by inspissation for 15 minutes at 80 to 90°C or steaming at 100°C for 10-15 minutes. |
| Storage | BioScien Loeffler Serum Medium Base should be stored between 10-30°C in a firmly closed container and the prepared medium at 2-8°C. Use before expiry date on the label. On opening, product should be properly stored dry, after tightly capping the bottle in order to avoid lump development due to the hygroscopic nature of the product. Improper storage of the product may lead to lump formation. Store in a dry ventilated area protected from extremes of temperature and sources of ignition. Seal the container tightly after use. Product performance is best if used within stated expiry period. |
| Appearance | The color of BioScien Loeffler Serum Medium Base is Light yellow to brownish yellow homogeneous free flowing powder. Basal medium: Light amber coloured clear solution. After addition of horse serum and coagulation: Off-white coloured opalescent slants forms in tubes. If there are any physical changes for powder or signs of deterioration (shrinking, cracking, or discoloration), and contaminations for hydrated media, discard the medium. |
| Specimen | Clinical samples: Tissue, Nasal and throat samples. |
| Pack sizes | 3 options |
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