Bacteriology Media
Brilliant Green Broth
Brilliant Green Broth is used as a selective differential medium for primary screening of Salmonella in polluted water using membrane filter technique.
| Range | Bacteriology Media |
|---|---|
| Composition | Formula per liter of medium: Peptone 20.00 gm; Yeast extract 6.000 gm; Lactose 20.00 gm; Saccharose 20.00 gm; Sodium chloride 10.00 gm; Phenol red 0.160 gm; Brilliant green 0.025 gm |
| Preparation | Suspend 76.19 grams in 1000 ml distilled water. Heat if necessary to dissolve the medium completely. DO NOT AUTOCLAVE. Cool to 35°C and saturate sterile absorbent cotton pad with 2 ml of the broth. The medium should be used within 24 hours of rehydration. |
| Storage | BioScien BRILLIANT GREEN BROTH should be stored between 10-30°C in a firmly closed container and the prepared medium at 2-8°C. Use before expiry date on the label. On opening, product should be properly stored dry, after tightly capping the bottle in order to avoid lump development due to the hygroscopic nature of the product. Improper storage of the product may lead to lump formation. Store in a dry ventilated area protected from extremes of temperature and sources of ignition. Seal the container tightly after use. Product performance is best if used within stated expiry period. |
| Appearance | The color of BioScien BRILLIANT GREEN BROTH is Light yellow to pink homogeneous free flowing powder. Prepared medium is greenish brown coloured clear to slightly opalescent solution. If there are any physical changes for powder or signs of deterioration (shrinking, cracking, or discoloration), and contaminations for hydrated media, discard the medium. |
| Specimen | Food and dairy samples; water samples |
| Pack sizes | 3 options |
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