Bacteriology Media

Lauryl Sulphate Broth (Lauryl Tryptose Broth)

Recommended for detection and enumeration of coliform bacteria in water, waste water, dairy products, and other food samples.

Range	Bacteriology Media
Composition	Formula per liter of medium: Tryptose 20.00 gm; Lactose 5.00 gm; Sodium chloride 5.00 gm; Dipotassium hydrogen phosphate 2.750 gm; Potassium dihydrogen phosphate 2.750 gm; Sodium lauryl sulphate (SLS) 0.100 gm; Final pH 6.8±0.2 at 25°C
Preparation	Suspend 35.60 grams in 1000 ml purified/distilled water. Heat if necessary to dissolve the medium completely. Distribute into tubes containing inverted Durhams tubes. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes. For inoculum of 1 ml or less, use single strength medium. For inocula of 10 ml or more, double strength or proportionate medium should be prepared.
Storage	BioScien Lauryl Sulphate Broth should be stored between 10-30°C in a firmly closed container and the prepared medium at 15-25°C. Use before expiry date on the label. On opening, product should be properly stored dry, after tightly capping the bottle in order to avoid lump development due to the hygroscopic nature of the product. Improper storage of the product may lead to lump formation. Store in a dry ventilated area protected from extremes of temperature and sources of ignition. Seal the container tightly after use. Product performance is best if used within stated expiry period.
Appearance	The color of BioScien Lauryl Sulphate Broth is Cream to yellow homogeneous free flowing powder. Prepared Media is Light yellow coloured, clear solution without any precipitate. If there are any physical changes for powder or signs of deterioration (shrinking, cracking, or discoloration), and contaminations for hydrated media, discard the medium.
Specimen	Food and dairy samples; Water samples.
Pack sizes	3 options