Bacteriology Media

LB Agar

Recommended for the cultivation and maintenance of recombinant strains of Escherichia coli for genetic and molecular biology studies; may be used for routine cultivation and estimation of not particularly fastidious microorganisms.

Range	Bacteriology Media
Composition	g/L: Peptone 10 g; Yeast Extract 5 g; Sodium Chloride 5 g; Agar 12 g
Preparation	Hydrated LB Agar media is prepared by suspend 32 grams of the medium in one liter of distilled water. Mix well and dissolve by heating with frequent agitation. Boil for one minute until complete dissolution. Sterilize in autoclave at 121°C for 15 minutes. Adjust the pH to 7.5±0.2, Pour into sterile petri dishes.
Storage	BioScien LB Agar dehydrated media are stable until expiration date stated on label when properly stored 10-30°C. Store between 10-30°C in a tightly closed container and the prepared medium at 20-30°C. Use before expiry date on the label. On opening, product should be properly stored dry, after tightly capping the bottle in order to prevent lump formation due to the hygroscopic nature of the product. Improper storage of the product may lead to lump formation. Store in dry ventilated area protected from extremes of temperature and sources of ignition. Seal the container tightly after use.
Appearance	The color of BioScien LB Agar medium is cream to yellow homogeneous free flowing powder, dehydrated medium is clear light amber coloured to slightly opalescent gel. After addition of 4.0% w/v aqueous solution at 25°C. pH: 7.5±0.2. If there are any physical changes for powder or signs of deterioration (shrinking, cracking, or discoloration), and contaminations for hydrated media, discard the medium.
Specimen	Recombinant strains of E.coli
Pack sizes	3 options