Arena BioScien
Bacteriology Media
Brilliant Green Agar
BS.1/BGA01.100.0100
Fits your perfection
Bacteriology Media
Brilliant Green Agar
Selective and differential medium for the isolation of Salmonella species other than S. typhi and S. paratyphi from clinical specimens.
| Range | Bacteriology Media |
|---|---|
| Composition | Formula per liter of medium: Proteose peptone 10.00 gm; Yeast extract 3.000 gm; Sucrose 10.00 gm; Lactose 10.30 gm; Brilliant Green 0.0125 gm; Sodium chloride 5.000 gm; Phenol red 0.080 gm; Agar 12.00 gm; pH 6.9±0.2 at 25 °C |
| Preparation | Suspend 50g in 1 liter of distilled water. Bring to the boil to dissolve completely. Sterilize by autoclaving at 121°C for 15 minutes. Cool to 50°C. Mix well and pour into sterile Petri dishes. Procedure: 1. Allow medium to reach room temperature. 2. Inoculate the specimen (fecal sample or rectal swab) in one corner of the plate and streak as to obtain isolated colonies. 3. A non-selective medium should also be inoculated to ensure recovery of low levels of gram-negative bacteria and characterize other organisms that may be present. 4. Incubate aerobically at 35°C. 5. Examine after 18-24 hours. 6. If no growth is observed re-incubate plates an additional 24 hours. |
| Storage | BioScien BRILLIANT GREEN Agar should be stored between 10-30°C in a firmly closed container and the prepared medium at 2-8°C. Use before expiry date on the label. On opening, product should be properly stored dry, after tightly capping the bottle in order to avoid lump development due to the hygroscopic nature of the product. Improper storage of the product may lead to lump formation. Store in a dry ventilated area protected from extremes of temperature and sources of ignition. Seal the container tightly after use. Product performance is best if used within stated expiry period. |
| Appearance | The color of BioScien BRILLIANT GREEN Agar is Light yellow to light pink homogeneous free flowing powder. Prepared medium is Greenish brown clear to slightly opalescent gel. If there are any physical changes for powder or signs of deterioration (shrinking, cracking, or discoloration), and contaminations for hydrated media, discard the medium. |
| Specimen | Clinical: faeces; Foodstuffs; Water samples; Pharmaceutical samples |
| Pack sizes | 3 options |
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