Bacteriology Media
Brilliant Green Bile Broth 2%
This medium is widely recommended for identifying and confirming the presence of coliform bacteria in water, wastewater, food, milk, and dairy products.
| Range | Bacteriology Media |
|---|---|
| Composition | Formula per liter of medium: Peptone 10.00 gm; Lactose 10.00 gm; Bile 20.00 gm; Brilliant green 0.0133 gm; Final pH (at 25°C) 7.2 ± 0.2; ** Formula adjusted, standardized to suit performance parameters. |
| Preparation | Suspend 40.01 grams in 1000 ml purified/distilled water. Heat if necessary to dissolve the medium completely. Distribute in fermentation tubes containing inverted Durhams tubes and sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes. For preparation of double strength it is recommended to heat the dissolved broth (80.02 grams per litre) at 100°C for 30 minutes. Do not autoclave double strength broth. |
| Storage | BioScien Brilliant Green Bile Broth 2% Store between 10-30°C in a tightly closed container and the prepared medium at 15-25°C. Use before expiry date on the label. On opening, product should be properly stored dry, after tightly capping the bottle in order to prevent lump formation due to the hygroscopic nature of the product. Improper storage of the product may lead to lump formation. Store in dry ventilated area protected from extremes of temperature and sources of ignition. Seal the container tightly after use. Product performance is best if used within stated expiry period. |
| Appearance | The color of BioScien Brilliant Green Bile Broth 2% is Cream to pale green coloured homogeneous free flowing powder. Prepared Medium, Emerald green coloured, clear solution without any precipitate. If there are any physical changes for powder or signs of deterioration (shrinking, cracking, or discoloration), and contaminations for hydrated media, discard the medium. |
| Pack sizes | 3 options |
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