Bacteriology Media
Lauryl Sulphate Agar
Used for enumeration of Escherichia coli and coliforms in water, using membrane filter technique.
| Range | Bacteriology Media |
|---|---|
| Composition | Formula per liter of medium: Peptic digest of animal tissue 39.00 gm.; Yeast extract 6.000 gm.; Lactose 30.00 gm.; Sodium Lauryl sulphate 1.000 gm.; Phenol red 0.200 gm.; Agar 15.00 gm.; Final pH 7.4 ± 0.2 at 25°C |
| Preparation | Suspend 91.2 grams in 1000 ml distilled water. Adjust pH to 7.4 ± 0.2 at 25°C. Heat to boiling to dissolve the medium completely. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes. |
| Storage | BioScien Lauryl Sulphate Agar should be stored between 10-30°C in a firmly closed container and the prepared medium at 2-8°C. Use before expiry date on the label. On opening, product should be properly stored dry, after tightly capping the bottle in order to avoid lump development due to the hygroscopic nature of the product. Improper storage of the product may lead to lump formation. Store in a dry ventilated area protected from extremes of temperature and sources of ignition. Seal the container tightly after use. Product performance is best if used within stated expiry period. |
| Appearance | The color of BioScien Lauryl Sulphate Agar is Light yellow to pink homogeneous free flowing powder. If there are any physical changes, discard the medium. Prepared medium is Red coloured clear to slightly opalescent gel forms in Petri plates, media should not be used if there are any signs of deterioration (shrinking, cracking, or discoloration), and contaminations. |
| Specimen | Clinical samples: faeces |
| Pack sizes | 3 options |
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