Bacteriology Media

Lactose Broth

A broth medium that is recommended for lactose fermentation studies and detection of coliform bacteria.

RangeBacteriology Media
CompositionFormula per liter of medium: Peptone 5 gm; Beef Extract 3 gm; Lactose 5 gm; Final pH 6.9±0.2 at 25°C
PreparationSuspend 13 grams in 1000 ml distilled water, mix well. Heat if necessary to ensure complete solution is prepared. Distribute in tubes containing inverted Durham's tubes. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes.
StorageBioScien Lactose Broth should be stored between 10-30°C in a firmly closed container and the prepared medium at 2-8°C. Use before expiry date on the label. On opening, product should be properly stored dry, after tightly capping the bottle in order to avoid lump development due to the hygroscopic nature of the product. Improper storage of the product may lead to lump formation. Store in a dry ventilated area protected from extremes of temperature and sources of ignition. Seal the container tightly after use. Product performance is best if used within stated expiry period.
AppearanceThe color of BioScien Lactose Broth is cream to yellow colored homogeneous free flowing powder. Prepared Media is light amber in color without any precipitate. If there are any physical changes for powder or signs of deterioration (shrinking, cracking, or discoloration), and contaminations for hydrated media, discard the medium.
SpecimenWater samples, food samples and dairy products.
Pack sizes3 options

Ordering information

Cat. No. (REF) Pack size Quote
BS.1/LC01.100.0100 100 Grams Quote
BS.1/LC01.250.0250 250 Grams Quote
BS.1/LC01.500.0500 500 Grams Quote
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