Bacteriology Media

Kovac’s Reagent

Detection of indole produced due to the breakdown of tryptophan by certain types of bacteria that produce tryptophanase.

RangeBacteriology Media
CompositionFormulation per 100 ml: p-Dimethylaminobenzaldehyde 5.00 gm; Isoamyl Alcohol 75.00 ml; Hydrochloric Acid 25.0 ml
ProcedureAdd 0.2 - 0.3 ml of Kovac's reagent to 5 ml of a 24 - 48 hours old culture of the organism under examination. Observe the formation of a red colored ring which indicates positive indole test within 1-3 minutes.
StorageBioScien Kovac’s Reagent is stable until expiration date stated on label when properly stored at 2-8°C away from light.
AppearanceThe color of BioScien Kovac’s Reagent is greenish yellow colored solution. It’s immiscible with water and should be clear with no insoluble particles.
SpecimenSample consists of bacterial growth obtained from a clinical sample or water sample.
Pack sizes3 options

Ordering information

Cat. No. (REF) Pack size Quote
BS.1/KR01.050.0050 50 ml Quote
BS.1/KR01.100.0100 100ml Quote
BS.1/KR01.500.0500 500 ml Quote
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Bacteriology Media

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