Bacteriology Media
Brain Heart Infusion (BHI) Broth
Designed for propagation of pathogenic cocci and other fastidious organisms associated with blood culture work and allied pathological investigations and for enrichment of Staphylococcus aureus.
| Range | Bacteriology Media |
|---|---|
| Composition | Formula per liter of medium: Glucose (Dextrose) 2.00 gm; Sodium chloride 5.00 gm; Disodium hydrogen phosphate 2.5 gm; BHI Powder * 5.00 gm; Peptone 10.0; HM infusion, solids # 12.5. Final pH 7.3 ± 0.2. * Equivalent to Dehydrated beef heart infusion. # Equivalent to Dehydrated calf brain infusion. ** Formula adjusted, standardized to suit performance parameters. |
| Preparation | Suspend 37.0 gram in 1000 ml purified / distilled water. Heat if necessary to dissolve the medium completely. Dispense into bottles or tubes as desired. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes. For best results, the medium should be used on the day it is prepared, otherwise, it should be boiled or steamed for a few minutes and then cooled before use. |
| Storage | BioScien BHI Broth Store between 10-30°C in a tightly closed container and the prepared medium at 15-25°C. Use before expiry date on the label. On opening, product should be properly stored dry, after tightly capping the bottle in order to prevent lump formation due to the hygroscopic nature of the product. Improper storage of the product may lead to lump formation. Store in dry ventilated area protected from extremes of temperature and sources of ignition. Seal the container tightly after use. Product performance is best if used within stated expiry period. |
| Appearance | The color of BioScien BHI Broth is Cream to yellow homogeneous free flowing powder. Prepared medium Light amber coloured, clear to slightly opalescent solution. |
| Pack sizes | 3 options |
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